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Tosoh: The Chemistry of Innovation
Hydrophobic Interaction Chromatography
HPLC Columns
Affinity
Hydrophobic Interaction
TSKgel Butyl-NPR
TSKgel Ether-5PW
TSKgel Phenyl-5PW
Ion Exchange
Normal Phase / HILIC
Reversed Phase
Size Exclusion
Parts and Accessories


Of the three TSKgel HIC columns, TSKgel Ether-5PW has intermediate hydrophobity. It is an excellent choice for separating hydrophobic molecules such as membrane proteins or monoclonal antibodies such as IgG or IgM.  Featuring a 100nm pore size in a polymethacrylate base material, the Ether-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.

 

Structure:

Product Attributes:

 Pore size (mean): 100nm
 Particle size (mean): 10, 13 and 20µm
 pH stability:  2.0 - 12.0
 Functional group:  Ether



Literature:

Catalog -- Chromatography Catalog, HIC Columns

Instruction Manual -- Guardgel Kits for TSKgel HPLC Columns

Operating Conditions and Specifications

Separation Report -- Separation of Monoclonal Antibodies Using TSKgel HPLC Columns

Separation Report -- Application of TSKgel Semi-Micro Columns for High-Sensitivity Analyses

 Applications


 Screening of mouse monoclonal antibodies


 Monoclonal antibody purified on TSKgel Ether-5PW



 

P/N
Description
Particle
size
Housing material ID
(mm)
Length
(cm)
0014013
TSKgel Ether-5PW
10μm
Glass 5 5
0014014
TSKgel Ether-5PW                            
10μm
Glass 8 7.5
0014025
Guardgel Kit for P/Ns 0014013 and 0014014 Glass
0018760
TSKgel Ether 5PW        
10μm
Stainless Steel 2 7.5 
0042156
Guard Cartridge for P/N 0018760, pk 3 
 
Stainless Steel  2 1
0019308
Guard Cartridge Holder for P/N 0042156 Stainless Steel  2 1
0008641
TSKgel Ether-5PW
10μm
Stainless Steel 7.5 7.5
0008643
Guardgel Kit for P/N 0008641 Stainless Steel


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Frequently asked questions -- HIC

 

Search the Tosoh HPLC Application Database


Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp