Home  
  Products  
  Literature/Applications  
  Service/Support  
  Search  
search
Tosoh: The Chemistry of Innovation
Hydrophobic Interaction Chromatography
HPLC Columns
Affinity
Hydrophobic Interaction
TSKgel Butyl-NPR
TSKgel Ether-5PW
TSKgel Phenyl-5PW
Ion Exchange
Normal Phase / HILIC
Reversed Phase
Size Exclusion
Parts and Accessories


The most hydrophobic among the three TSKgel HIC columns, TSKgel Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules. Featuring a 100nm pore size in a polymethacrylate base material, the Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.

Stainless steel is the standard column format for TSKgel Phenyl-5PW. Glass and PEEK column housings are available for samples that may adsorb to stainless steel. The alternative column formats are also suitable for general applications.

 

Structure:

Phenyl-5PW Chemical Structure

 

Product Attributes:

 Pore size (mean): 100nm
 Particle size (mean): 10, 13 and 20µm
 pH stability:  2.0 - 12.0
 Functional group:  Phenyl



Literature:

Catalog -- Chromatography Catalog, HIC Columns

Instruction Manual -- Guardgel Kits for TSKgel HPLC Columns

Operating Conditions and Specifications

Separation Report -- Separation of Monoclonal Antibodies Using TSKgel HPLC Columns

Separation Report -- Application of TSKgel Semi-Micro Columns for High-Sensitivity Analyses

 Applications


 Retain large RNAs on TSKgel Phenyl-5PW


 Scale up to preparative separations




 
P/N
Description
Particle
size
Housing material ID
(mm)
Length
(cm)
0013063
TSKgel Phenyl-5PW
10µm
Glass 5  5
0008804
TSKgel Phenyl-5PW
10µm
Glass 8 7.5
0018759
TSKgel Phenyl-5PW
10µm
Stainless Steel 2 7.5
0042155
Guard  Cartridge for P/N 0018759
 
Stainless Steel 2 1
0019308
Guard Cartridge Holder for P/N 0042155 Stainless Steel 2 1
0007573
TSKgel Phenyl-5PW
10µm
Stainless Steel 7.5 7.5
0007652
Guardgel Kit for P/N  0007573
 
Stainless Steel
0007656
TSKgel Phenyl-5PW
13µm
Stainless Steel 21.5 15
0016095
Guardgel Kit for P/N 0007656   Stainless Steel    
 0007938 TSKgel Phenyl-5PW
20µm
Stainless Steel

55

20 
0007936
Guard Column for P/N 0007938
 
Stainless Steel

45

5
0020023
TSKgel BioAssist Phenyl
10µm
PEEK  7.8 5


Printer Friendly Version

 

Frequently asked questions -- HIC

 

Search the Tosoh HPLC Application Database


Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp