Home  
  Products  
  Literature/Applications  
  Service/Support  
  Search  
search
Tosoh: The Chemistry of Innovation
Ion Exchange Chromatography
Ion Exchange Chromatography
Anion Exchange
TSKgel BioAssist Q
TSKgel DEAE-2SW
TSKgel DEAE-3SW
TSKgel DEAE-5PW
TSKgel DEAE-NPR
TSKgel DNA-NPR
TSKgel DNA-STAT
TSKgel QAE-2SW
TSKgel Q-STAT
TSKgel SAX
TSKgel Sugar AXG
TSKgel Sugar AXI
TSKgel SuperQ-5PW
Cation Exchange


Anion exchange chromatography is practiced with either a strong or a weak anion exchange column, containing a quarternary ammonium ion, or with a weak anion exchanger, having either a tertiary or secondairy amine functional group, such as DEAE (diethylaminoethyl). A counter ion, often Cl- maintains electroneutrality.

In ion exchange chromatography the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For instance, in anion exchange chromatography a molecule with a pI of 6.8 is run in a mobile phase buffer at pH 8.0 with the solid support pKa at 10.3.

Speciality Column

  • Oligonucleotide fragments, PCR reagents (DNA-NPR)

Highlights:

  • Pore structure and bonding chemistry of BioAssist Q columns provides high capacity for small to very large molecular weight proteins and nucleic acids
  • TSKgel SuperQ-5PW columns have higher capacity than TSKgel DEAE-5PW due to novel bonding chemistry; effective pore size is smaller for SuperQ-5PW
  • Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is roughly double that of the 5PW packings due to the smaller pore size and larger surface area.
  • TSKgel DEAE-5PW and DEAE-2SW columns are available in 2mm ID format
  • Specialty columns for analysis of mono- and disaccharides, sugar alcohols, and organic acids.
  • TSKgel DNA-NPR columns for PCR fragment analysis
  • BioAssist columns are packed in PEEK hardware, while other columns are available in glass and stainless steel.
  • Very efficient chromatography can be achieved with the TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores. 


Strong Anion Exchange 

Weak Anion Exchange

Methacrylate

Silica: TSKgel QAE-2SW

Polymer: TSKgel Q-STAT

Polystyrene

Methacrylate

Silica

Polymer: TSKgel DNA-STAT

 


Return to Ion Exchange main page

 

-- NEW --

TSKgel Q-STAT
TSKgel DNA-STAT
Anion Exchange Columns

Packed with non-porous resin particles, these columns enable high speed and high resolution analysis, as well as isolation, of biomolecules.


Anion Exchange Chromatography
is performed with either a strong anion-exchange column, containing a quaternary ammonium ion, or with a weak anion-exchanger, having either a tertiary or secondary amine functional group, such as DEAE (diethylaminoethyl). A counter ion, often Cl-, maintains electroneutrality.

Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp