TSKgel BioAssist Q columns are packed with spherical, polymer beads with approx. 400nm pores. They are surface modified with a strong anion exchanger. The very large pores allow large biomolecules to freely enter and exit the pores resulting in higher binding capacity and better recovery of activity than other competivie materials. The standard column is 4.6mm ID x 5cm, while a 10mm ID x 10cm semi-preparative column is available for scale-up. Both columns are made of PEEK to reduce protein adsorption.

Product Attributes:

Matrix:                polymethacrylate  Particle size (mean): 10 and 13 µm  Pore size (mean): 400nm  Functional group: polyamine  Counter ion: Cl-  pH range: 2.0 -12.0  Capacity (g BSA/L): 70  Small ion capacity: 0.1eq/L  pKa: 9.4

Literature:

Application Note -- Ion Exchange Analysis of Monoclonal Antibodies on Large Pore TSKgel BioAssist Columns

Catalog -- Chromatography Catalog, IEC Columns, Overview

Catalog -- Chromatography Catalog, IEC Columns, Anion

Operating Conditions and Specifications

Product Overview -- TSKgel BioAssist Ion Exchange Columns

Separation Report - TSKgel BioAssist Series Ion Exchange Columns

Technical Presentation -- Purification of Immunoglobulin M using a novel ion-exchanger

Technical Presentation -- The Characteristics of Novel Semi-Preparative Ion-Exchange Columns

Technical Presentation -- Fast Protein Separations with Ion Exchange Columns

 

P/N Description Particle size Housing material ID (mm) Length (cm) 0019685 TSKgel BioAssist Q 10µm PEEK 4.6 5 0021410 TSKgel BioAssist Q 13µm PEEK 10 10