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Tosoh: The Chemistry of Innovation
Ion Exchange Chromatography
HPLC Columns
Anion Exchange
TSKgel BioAssist Q
TSKgel DEAE-2SW
TSKgel DEAE-3SW
TSKgel DEAE-5PW
TSKgel DEAE-NPR
TSKgel DNA-NPR
TSKgel DNA-STAT
TSKgel QAE-2SW
TSKgel Q-STAT
TSKgel SAX
TSKgel Sugar AXG
TSKgel Sugar AXI
TSKgel SuperQ-5PW
Cation Exchange


TSKgel DNA-NPR
columns are packed with 2.5 micron, non-porous, high efficiency, hydrophilic polymer beads which are surface modified with a weak anion exchanger. Because NPR columns contain non-porous particles, binding capacity is low compared to porous columns with the same ligand functionality.

Column dimensions are optimized for the high efficiency separation of DNA fragments, PCR products, or plasmids. A small guard column is available to protect the analytical column.

 

Product Attributes:

 Matrix: hydroxylated methacrylic polymer
 Particle size (mean): 2.5µm
 Pore size (mean): non-porous
 Functional group: proprietary
 Counter ion: ClO4-
 pH range: 2-12
 Capacity (g BSA/L): 5
 Small ion capacity: >0.1 eq/L
 pKa: 11.2



Literature:

Application Note -- Purification of a 13-mer DNA Phosphorothioated Crude Deprotected Oligonucleotide by Strong Anion Exchange Chromatography Using TSKgel DNA- NPR

Catalog -- Chromatography Catalog, IEC Columns, Overview

Catalog -- Chromatography Catalog, IEC Columns, Anion

Operating Conditions and Specifications

Product Overview -- TSKgel DNA-NPR Anion Exchange Columns

Application Overview -- Purification of Nucleic Acids

 

 Applications


Plasmid analysis with TSKgel DNA-NPR column



 

 

P/N
Description
Particle
size
Housing material ID
(mm)
Length
(cm)
0018249

TSKgel DNA-NPR

2.5µm
Stainless Steel 4.6 7.5
0018253
Guard Column  for P/N 0018249   5 Stainless Steel 4.6 0.5


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