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Tosoh: The Chemistry of Innovation
Ion Exchange Chromatography
HPLC Columns
Anion Exchange
Cation Exchange
TSKgel BioAssist S
TSKgel CM-2SW
TSKgel CM-3SW
TSKgel CM-5PW
TSKgel CM-STAT
TSKgel OApak-A
TSKgel SCX
TSKgel SP-2SW
TSKgel SP-5PW
TSKgel SP-NPR
TSKgel SP-STAT


TSKgel CM-STAT columns are packed with 7 and 10µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered weak cation exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity, low operating pressure and rapid analysis.

Applications for the TSKgel CM-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.

 

Product Attributes:

 Matrix:   hydrophilic polymer
 Particle size (mean): 7 and 10µm
 Pore size (mean): non-porous
 Functional group: carboxymethyl
 Counter ion: Na
 pH range: 3.0 - 10.0
 Static binding 
 capacity (mg 
 Lysozyme/g dry gel):
ca. 20 (7µm)
ca. 15 (10µm)
 Small ion capacity: 100ueq/g dry gel
 pKa: 4.9



Literature:

Technical Presentation -- High Speed and High Resolution Cation Exchange Chromatography for Biological Samples on Non-Porous Packings 

Operating Conditions and Specifications

Product Overview -- TSKgel CM-STAT Cation Exchange Columns

 

 Applications


 The analysis profiles for five antibodies on a TSKgel CM-STAT column were compared with the profiles obtained on a competitive non-porous cation exchange column. Similar or higher resolution profiles were obtained on the TSKgel CM-STAT column in approximately half the time.


TSKgel CM-STAT, 4.6mm ID x 10cm

ProPac WCX-10, 4mm ID x 25cm

 


 
P/N
Description
Particle
size
Housing material ID
(mm)
Length
(cm)
0021965
TSKgel CM-STAT
10µm
Stainless Steel 3 3.5
0021966
TSKgel CM-STAT
7µm
Stainless Steel 4.6 10


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Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp