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Tosoh: The Chemistry of Innovation
Ion Exchange Chromatography
HPLC Columns
Anion Exchange
Cation Exchange
TSKgel BioAssist S
TSKgel CM-2SW
TSKgel CM-3SW
TSKgel CM-5PW
TSKgel CM-STAT
TSKgel OApak-A
TSKgel SCX
TSKgel SP-2SW
TSKgel SP-5PW
TSKgel SP-NPR
TSKgel SP-STAT


TSKgel SP-STAT columns are packed with 7 and 10µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered cation exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity and a low operating pressure.

Applications for the TSKgel SP-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.

 

Product Attributes:

 Matrix:   hydrophilic polymer
 Particle size (mean): 7 and 10µm
 Pore size (mean): non-porous
 Functional group: sulfopropyl
 Counter ion: Na
 pH range: 3.0 - 10.0

Static binding
capacity (mg 
Lysozyme/g dry
 gel):

ca. 15 (7µm)
ca. 10 (10µm) 
 Small ion capacity: 23ueq/g dry gel
 pKa: 4.0



Literature:

Technical Presentation -- High Speed and High Resolution Cation Exchange Chromatography for Biological Samples on Non-Porous Packings 

Operating Conditions and Specifications

Product Overview -- TSKgel SP-STAT Cation Exchange Columns

 

 Applications


Separation of nucleosides by ion-exchange chromatography on TSKgel SP-2SW


Conversion ratio of peak area

 


 
P/N
Description
Particle
size
Housing material ID
(mm)
Length
(cm)
0021963
TSKgel SP-STAT
10µm
Stainless Steel 3 3.5
0021964
TSKgel SP-STAT
7µm
Stainless Steel 4.6 10

 


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Frequently asked questions -- IEC

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Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp