TSKgel Amide-80 columns are packed with 3 (new), 5, or 10µm spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3µm columns offer shorter analysis time and enhanced sensitivity for LC-MS applications. At any particle size, the stability of the TSKgel Amide-80 bonded phase columns is superior over conventional amino-bonded phase columns.
Bonded phase pore size is indicated by the number in the product description, in this case TSKgel Amide-80 has 8nm nominal pore size. The pore size of the base silica is 10nm. |