TSKgel Phenyl-5PW RP offers similiar selectivity to its hydrophobic interation counterpart TSKgel Phenyl-5PW, although a higher degree of cross-linking allows for increased stability in aqueous/organic mobile phase gradients.

The highly cross-linked polymethacrylate base material provides an advantage over silica when high pH buffer systems are needed. Additionally, TSKgel Phenyl-5PW RP can withstand rigorous cleaning protocols using either acid or base. The 100nm pore size accommodates globular protein samples up to 1,000,000 Da.

Structure:

Product Attributes:

Pore size (mean): 100nm  Exclusion Limit: 1,000,000 Da  Estimated ligand density: 1eq/L  Particle size (mean): 10, 13 and 20 μm  pH stability: 2.0 -12.0  Functional group: Phenyl (monomeric bonding chemistry)

Literature:

Brochure -- Reversed Phase and HILIC TSKgel HPLC Columns

Catalog -- Chromatography Catalog, Traditional Reversed Phase Columns

Operating Conditions and Specifications

 

P/N Description Particle size Housing material ID (mm) Length (cm) 0014007 TSKgel Phenyl-5PW RP 10μm Glass 8 7.5 0018756 TSKgel Phenyl-5PW RP 10μm Stainless Steel 2 7.5 0042159 Guard Cartridge for P/N 0018756 10μm Stainless Steel  2 1   0019308 Guard Cartridge Holder for P/N 0042159   Stainless Steel 2 1 0008043 TSKgel Phenyl-5PW RP 10μm Stainless Steel 4.6 7.5 0019007 Guard Cartridges for P/N 0008043, 3 pk, Replaces P/N 0014126 10μm Stainless Steel 3.2 1.5  0019018 Guard Cartridge Holder for P/N 0019007, Replaces P/N 0014100   Stainless Steel 3.2 1.5  0016260 TSKgel Phenyl-5PW RP 13μm Stainless Steel 21.5 15