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Tosoh: The Chemistry of Innovation
Reversed Phase Columns: Silica-based
HPLC Columns
8nm Monomeric RP Silica
10nm Monomeric RP Silica
2µm Super Series RP Silica
Specialty RP Silica
High Throughput RP Silica
TSKgel ODS-140HTP


TSKgel ODS-140HTP columns provide high resolution and short analyses time at moderate pressures, enabling high throughput separations.  The polylayer bonding chemistry of the 2.3µm particle size of these columns results in highly efficient and durable columns.  The lower pressure drop reduces the burden on the hardware, allowing the TSKgel ODS-140HTP columns to be used with either UPLC® (up to 9000psi) or conventional HPLC systems.

For use in high throughput applications, including drug discovery, pharmacokinetics and peptide digest separations, TSKgel ODS-140HTP columns offer excellent peak shape for basic compounds.

 

Structure:


Product Attributes:

 Pore size (silica): 14nm
 Endcapped: yes
 Particle size: 2.3µm
 pH stability: 2.0 - 7.5
 Functional group:  C18 (polymeric bonding chemistry)
 % carbon 8%



Literature:

Application Note -- TSKgel ODS-140HTP, 2.3µm: Ultra High Performance Reversed Phase Columns for High Throughput Analysis

Brochure -- Reversed Phase and HILIC TSKgel HPLC Columns

Catalog -- Chromatography Catalog, Reversed Phase Columns

Operating Conditions and Specifications

Product Overview -- TSKgel ODS-140HTP Columns

 Performance Data


Column stability:

TSKgel ODS-140HTP columns are stable at high flow rates under demanding step gradient conditions. 

Figure A shows that consistent theoretical plate values were obtained on the TSKgel ODS-140HTP column during 1110 gradient cycles consisting of five minute step gradients from 10% to 50% and from 50% to 100% methanol at 0.6mL/min.  During each cycle, pressure fluctuated between 30 and 60MPa.  

Figure A:

Figure B shows injections of test solutes after the first step gradient cycle and after 1110 cycles.  The results clearly demonstrate the durability of the TSKgel ODS-140HTP columns when operated at high flow rate and high pressure.

Figure B:



P/N
Description
Particle
size
Housing material ID
(mm)
Length
(cm)
0021927
TSKgel ODS-140HTP
2.3μm
Stainless Steel 2.1 5
0021928
TSKgel ODS-140HTP
2.3μm
Stainless Steel

2.1  

10 


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Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp