TSKgel ODS-80Ts QA columns were developed specifically for use by QA/QC departments that require highly reproducible separations. These columns are prepared from the same endcapped C18 packing material as TSKgel ODS-80Ts columns, but with narrower manufacturing specifications to meet the demand for high reproducibility.
The variation between different lots of TSKgel ODS-80Ts QA packing material is minimized by selecting batches of TSKgel ODS-80Ts that fall within a very narrow range of specifications, as demonstrated in the table below. In addition, each column must pass demanding specifications for efficiency (N) and peak asymmetry, as are spelled out in the Operating Conditions and Specifications sheet. The end result is TSKgel ODS-80Ts QA columns that exhibit an unparalleled level of reproducibility for retention, selectivity (k´), efficiency (N), and peak symmetry.
Product Specifications:
|
Attribute |
Specification Range |
Lot-to-Lot Reproducibility (CV%) |
|
Particle size:
- Distribution (dp90/dp10) |
4.95 - 5.35
1.55 - 1.70 |
0.6
1.8 |
|
Surface area (m2/g) |
410 - 440 |
0.5 |
|
Pore size (mL/g) silica: |
9.0 - 10.0 |
0.5 |
|
Pore volume (nm):
|
0.96 - 1.04 |
0.7 |
|
Carbon content (wt%): |
14.0 - 15.0 |
N/A |
|
C18 coverage (µmol/m2): |
1.71 - 1.99 |
1.3 |
|
Metal ion content (ppm)
- Na
- Al
- Fe
- Ti |
<10
<10
<10
<10
|
N/A
N/A
N/A
N/A
| |
Column classification:
TSKgel ODS-80Ts QA columns were submitted to several characterization tests to determine the level of hydrophobic retention, steric selectivity and retention of basic compounds. The results of the characterization tests were used to establish specifications listed in the Table below.
|
Parameter |
|
CV (%) |
Test Conditions |
|
k' naphthalene (hydrophobicity) |
|
1.3 |
1 |
|
α triphenylene/o-terphenyl (steric selectivity) |
|
0.4 |
1 |
|
k' procainamide (basic compounds) |
|
2.6 |
2 |
|
k' phenol
|
|
0.9 |
2 |
|
k' oxine copper (inertness to chelating compounds) |
|
3.5 |
3 | |
1. 80% CH3OH/20% H2O, T=40°C
2. 10% ACN/90% 20mmol/L Na2HPO4 (pH6.6, adjusted with 20mmol/L NaH2PO4, T=40°C)
3. 7% ACN/93% 20mmol/L H3PO4, T=40°C
|
Application
To demonstrate the lot-to-lot reproducibility that can be expected when using TSKgel ODS-80Ts QA columns, the contents of an over-the-counter analgesic pain reliever was analyzed on columns from three different lots of packing material. The results are shown in the Figure to the right and Table below. Excellent reproducibility of retention, peak height, peak area, efficiency and peak shape is evident for all three ingredients.
Highly reproducible results can be achieved using the TSKgel ODS-80Ts QA columns as shown in this lot-to-lot reproducibility test. |
|
Analysis of an over-the-counter analgesic
pain reliever
| | |
Results
|
Compound |
Lot# |
RT (min) |
Peak Area
(mv x sec) |
Peak Height |
N |
AF |
|
caffeine |
|
3.047
3.080
3.080 |
4.51575 x 102
4.50235 x 102
4.44175 x 102 |
99.26
94.04
95.63 |
10,396
9,597
10,143 |
1.10
1.13
1.13 |
|
salicylamide |
|
6.190
6.253
6.250 |
3.96172 x 102
3.94822 x 102
3.90617 x 102 |
54.06
52.23
52.40 |
16,441
15,797
16,333 |
1.03
1.07
1.06 |
|
acetylsalicylic acid |
|
9.983
10.080
10.063 |
4.34473 x 102
4.33835 x 102
4.27633 x 102 |
37.35
36.48
36.28 |
|
1.03
1.05
1.05 | |
Literature:
Brochure -- Reversed Phase and HILIC TSKgel HPLC Columns
Operating Conditions and Specifications
|
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
|
0018768 |
TSKgel ODS-80Ts QA |
5μm |
Stainless Steel |
2 |
15 |
|
0018769 |
TSKgel ODS-80Ts QA |
5μm |
Stainless Steel |
2 |
25 |
|
0018519 |
TSKgel ODS-80Ts QA |
|
Stainless Steel |
4.6 |
7.5 |
|
0018520 |
TSKgel ODS-80Ts QA |
|
Stainless Steel |
4.6 |
15 |
|
0018521 |
TSKgel ODS-80Ts QA |
|
Stainless Steel |
4.6 |
25 |
|
0019325 |
Guard Cartridge for 2mm ID columns, 3 pk |
|
Stainless Steel |
2 |
1 |
|
0019308 |
Guard Cartridge Holder for 2mm ID cartridges |
|
Stainless Steel |
2 |
1 |
|
0019011 |
Guard Cartridge for 4.6mm ID columns, 3 pk |
|
Stainless Steel |
3.2 |
1.5 |
|
0019018 |
Guard Cartridge Holder for 4.6mm ID cartridges |
|
Stainless Steel |
3.2 |
1.5 | |
|