Featuring trimethylsilane groups covalenty attached to large 25nm pores, TSKgel TMS-250 is an excellent choice for analysis of larger proteins by reversed phase HPLC. Due to the low hydrophobicity of the ligand, excellent recoveries are common even when used with large proteins. Proteins such as adolase (158kDa) exhibit sharp peaks relative to wide pore C18 columns.

Structure:

Product Attributes:

Pore size (mean): 25nm  Exclusion Limit: 200,000 Da  Endcapped: Yes  Particle size (mean): 10 µm  pH stability: 2.0 - 7.5  Functional group: C1 (monomeric bonding chemistry)  % carbon 5%

Literature:

Brochure -- Reversed Phase and HILIC TSKgel HPLC Columns

Catalog -- Chromatography Catalog, Traditional Reversed Phase Columns

Operating Conditions and Specifications

 

P/N Description Particle size Housing material ID (mm) Length (cm) 0007190 TSKgel TMS-250 10μm Stainless Steel 4.6 7.5