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The TSKgel SuperH column line consists of eight columns with different pore sizes, TSKgel SuperH1000 through TSKgel SuperH7000, and four columns with an extended linear range of the calibration curve. The calibration curves are shown below.
SuperH-type columns are high efficiency/high throughput versions of the conventional HHR columns. Both column types are based on the same bead chemistry.
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Calibration curves on TSKgel SuperH columns
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Main characteristics
- Maximum operating temperature for SuperH-type columns is 140°C.
- Broad solvent range. All SuperH-type columns are shipped in THF, which can be exchanged for a wide variety of organic solvents, as is shown below.
- SuperH-type columns are high efficiency, high-throughput gel permeation (HT-GPC) columns with dimensions of 6mm ID x 15cm.
Solvent compatibility for TSKgel SuperH and Hhr columns
The column shipping solvent limits the types of solvents that can be used with each column type.
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Column type |
Shipping solvent* |
Can be replaced with |
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SuperH and Hhr1 |
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acetone, ethanol, quinoline, benzene, o-dichlorobenzene, ethyl acetate, dodecane, FC-113, carbon tetrachloride5, dichloromethane, dichloroethane, trichloroethane, n-hexane, cyclohexane, xylene, tetrahydrofuran, chloroform, 1,4-dioxane, hexafluoroisopropanol, toluene, 1-chloronaphthalene, dimethylaceto acetamide, methyl ethyl ketone, trichlorobenzene, m-cresol, dimethylformamide, methylpyrrolidone, o-chlorophenol/chloroform, dimethyl sulfoxide, pyridine | |
Notes:
1) In case of SuperH and HHR, see table below for appropriate flow rates for solvent exchange. After switching to a very polar solvent, switching to a nonpolar is not recommended.
2) All TSKgel HXL, HHR, SuperHZ, and SuperH analytical columns are shipped containing tetrahydrofuran (THF), except High Temperature (HT) columns, which contain o-dichlorobenzene.
3) Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.
* 100% methanol cannot be used with H-type columns; use this solvent with TSKgel SW-type or Alpha-type columns.
** TSKgel H columns may be specially ordered with this shipping solvent.
SRecommended flow rates (mL/min) for solvent exchange in SuperH and Hhr columns
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Solvent |
SuperH (6.0mmIDx15cm) |
Hhr (7.8mmIDx30cm) |
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n-Hexane |
0.50 |
0.9 |
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methyl ethyl ketone |
0.40 |
0.7 |
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dichloromethane, ethyl acetate |
0.35 |
0.6 |
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toluene, chloroform |
0.30 |
0.5 |
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dimethylformamide |
0.20 |
0.4 |
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carbon tetrachloride, pyridine |
0.15 |
0.3 |
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dimethyl sulfoxide, dioxane, ethanol,
N-methylpyrrolidone, o-dichlorobenzene |
0.10 |
0.2 |
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quinoline, hexafluoroisopropanol,
1-chloronaphthalene |
0.05 |
0.1 | |
Recommended Solvents by Application for TSKgel H-type columns
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Recommended solvent |
Application |
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THF |
polystyrene, epoxy resin, phenoxy resin, polycarbonate, polyisoprene, polyvinyl acetate, polyvinyl chloride, monglycerides, fatty acids, poly (butadiene), polybutadiene, poly (methyl methacrylate), poly (styrene-butadiene), poly (styrene-acrylonitrile) |
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N,N-Dimethylformamide (DMF) |
polyvinyl chloride, polyvinyl fluoride, urea resins, polyurethane, polystyrene, polyester, polyimido ether, polyimido ester, polyphenol (aqueous solution), polyacrylonitrile |
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o-Dichlorobenzene (OCDB) |
polyethylene, polypropylene |
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Chloroform |
polycarboxylic ether, acrylic resin, epoxy resin, polystyrene |
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m-Cresol/Chloroform |
nylon, polyseter, polyamide, poly (ethylene terephthalate) |
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Toluene |
polybutadiene, polysiloxane | |
Beware of extra-column bandbroadening
The volume of a SuperH column is 3.4 times smaller than that of a conventional 7.8mm x 30cm column. As a result, peak volumes will be proportionally smaller on SuperH-type columns compared to a corresponding Hhr column. Thus, your HPLC system may require optimization of components that can give rise to extra-column bandbroadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.
Columns with linear calibration curves
The TSKgel SuperH product line contains four linear or mixed-bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixed-bed columns feature increasing linear calibration ranges, from TSKgel SuperHM-L, SuperHM-N, SuperHM-M, to SuperHM-H. |
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