TSKgel SW-type columns are silica-based. A hydrophilic diol-type bonded phase shields the silica surface from interacting with protein samples. Three different pore sizes are available. 12.5nm pore size for analysis of small proteins and peptides 25nm pore size for most protein samples 45nm pore size for very large proteins and nucleic acids Compared to competitive HPLC columns, SW-type columns contain more pore volume per unit column volume, which results in higher MW selectivity or better resolution. Particle sizes for the three SW-type column lines are: SuperSW: 4 micron SWXL: 5-8 micron SW: 10-13 micron See each of the SW-type product lines for representative calibration curves.

Highlights TSKgel SW-type packings are comprised of rigid spherical silica gel particles chemically derivatized with diol-containing ligands. Low adsorption and well-defined pore size distribution, which are requirements for high performance Gel Filtration of proteins. Three pore sizes are available in SW or SWXL product lines, two in the SuperSW product line  TSKgel SuperSW columns feature 4 micron particles for the highest resolution. The narrow 4.6mm column ID provides the highest sensitivity when limited in sample mass. Semi-preparative (21.5mm ID) and preparative (55mm, 108mm ID) stainless steel columns are available for precise scale-up to commercial production. PEEK and Glass column hardware are available in the SWXL product line.

TSKgel  SuperSW	TSKgel SWXL	TSKgel SW
TSKgel SuperSW columns contain spherical 4µm particles (12.5nm and 25nm only) packed in 30cm x 4.6mm ID columns for: For R&D. Use to benefit from high sensitivity when limited in sample mass. 2.6-fold reduction in solvent consumption. Use 5 or 8µm particle size TSKgel SWXL columns to improve sample resolution or to reduce analysis time (over SW columns). For QC of mAb. Use PEEK columns to reduce sample adsorption to stainless steel or glass. Analytical TSKgel SW column line features 10 or 13µm particle sizes for improved sample capacity. For protein isolation. Select from stainless steel or glass column hardware. Use preparative columns up to 55mm ID for larger scale purifcations.

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Application Overview -- Gel Filtration Chromatography with TSKgel columns