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TSKgel SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
Particle Characteristics
SW-type columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes.
Calibration Curves
The figure below shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SW-type columns. The following table summarizes the molecular weight ranges for G2000SW, G3000SW and G4000SW columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores. For comparative purposes, similar data is provided as well for TSKgel SWXL and SuperSW-type columns.
Polyethylene oxide, dextran and protein calibration curves for TSKgel SW columns
Properties and separation ranges for TSKgel SW-type packings (Molecular Weight in Da)
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TSKgel packing |
Particle
size
(µm) |
Pore
size (nm) |
Globular
proteins |
Dextrans |
Polyethylene
glycols and oxides |
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SuperSW2000 |
4 |
12.5 |
5,000 - 150,000 |
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G2000SWXL/
BioAssist G2SWXL |
5 |
12.5 |
5,000 - 150,000 |
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G2000SW |
10, 13 |
12.5 |
5,000 - 150,000 |
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SuperSW3000 |
4 |
25 |
10,000 - 500,000 |
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G3000SWXL/
BioAssist G3SWXL |
5 |
25 |
10,000 - 500,000 |
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G3000SW |
10 |
25 |
10,000 - 500,000 |
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G4000SWXL/
BioAssist G4SWXL |
8 |
45 |
20,000 -7,000,000 |
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G4000SW |
13, 17 |
45 |
20,000 -7,000,000 |
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Data generated using the following conditions:
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Columns: |
Two 4µm, 4.6mmIDx30cm TSKgel SuperSW columns in series; two 5µm, 7.8mmIDx30cm TSKgel SWXL columns in series; two 10µm, 7.5mmIDx60cm TSKgel SW columns in series |
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Elution: |
Globular proteins: 0.3M NaCl in 0.1M (0.05M for SWXL columns) phosphate buffer, pH 7
Dextrans and polyethylene glycols and oxides (PEOs): distilled water |
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Application Overview -- Gel Filtration Chromatography with TSKgel columns |