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Tosoh: The Chemistry of Innovation
Gel Filtration Chromatography
HPLC Columns
SW
SWXL
SuperSW
TSKgel SuperSW2000
TSKgel SuperSW3000


Featuring ultra-efficient 4μm particles, TSKgel SuperSW3000 provides higher efficiency than 5μm TSKgel G3000SWXL columns. The 25nm pore size results in a fractionation range up to 500,000 Da for globular proteins. The narrow 4.6mm column inner diameter reduces buffer consumption and is ideal for sample-limited applications. 

To fully benefit from the high efficiency of 4μm columns, and because the column diameter of TSKgel SuperSW columns is 4.6mm or less, it is important to minimize the amount of dead volume in the HPLC system. Consult the Separation Report for details (provided below).

 

Product Attributes:

 Base material: Silica
 Particle size (mean): 4µm
 Pore size (mean): 25nm
 Functional group: Diol
 pH stability: 2.5 - 7.5
 Calibration range: 10,000-500,000 Da(globular proteins)


Literature:

Application Note -- Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column

Application Note -- The Characterization of Biological Samples by Microbore TSKgel SuperSW3000 Size Exclusion Columns

Application Note -- Stability of TSKgel SuperSW3000 Columns for Analyzing Proteins by SEC

Application Overview -- Gel Filtration Chromatography with TSKgel columns

Catalog -- Chromatography Catalog, SEC Columns, Overview

Catalog -- Chromatography Catalog, SEC Columns, SW-type

Operating Conditions and Specifications

Product Overview -- TSKgel SuperSW2000 and SuperSW3000 columns

Separation Report --TSKgel SuperSW Series (rev. 10/07)

Technical Presentation -- High sensitivity protein separations using a new size exclusion chromatography microcolumn for use in conjunction with MS

 Applications


Overlays of monoclonal antibody separation with 0.2M NaCl, 0.2M NaClO4 and 0.4M NaClO4

(Enlargement of fusion protein 1's baseline region shows improved peak shape of the dimer and trimer aggregates with 0.4M NaClO4)

 

Published with permission of Scott Lauder, Lexigen Pharmaceuticals
 


 
P/N
Description
Particle
size
Housing material ID
(mm)
Length
(cm)
0018675
TSKgel SuperSW3000
4µm
Stainless Steel 4.6 30
0018762
Guard Column for P/N 0018765 Stainless Steel 4.6 3.5
0021485
TSKgel SuperSW3000
4µm
Stainless Steel 2 30
0021845
TSKgel SuperSW3000
4µm
Stainless Steel 1 30


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Frequently asked questions -- SEC

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Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp