TSKgel PWXL columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for PWXL columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSKgel PW page for typical samples and suitable eluents. Figure 1 shows the calibration curves for all PWXL-type columns. Figure 2 shows the benefits of PWXL columns over PW columns, namely the improved resolution due to the smaller particle sizes used in PWXL columns.
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Figure 1: Polyethylene glycol and oxide calibration curves on TSKgel PWXL columns
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Figure 2: Faster analysis and higher resolution with TSKgel PWXL columns
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Although proteins can be analyzed on PWXL columns, pore volumes are much smaller than on SW-type columns. Figure 3 demonstrates the limited separation power of PWXL for protein samples.
Specialty Columns
TSKgel G-DNA-PW is available for the analysis of DNA fragments. Similarly, TSKgel G-Oligo-PW , is the preferred column for the analysis of oligosaccharides. The mixed-mode column, TSKgel GMPWXL, has an extended linear calibration range, suitable for samples with a broad MW distribution and unknowns. |
Figure 3: Protein Calibration curves for TSKgel PWXL
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