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Tosoh: The Chemistry of Innovation
Process Media Products
Process Media Products
Ion Exchange (IEC)
Hydrophobic Interaction (HIC)
Affinity (AFC)
Toyopearl AF-Amino-650
Toyopearl AF-Blue HC-650
Toyopearl AF-Carboxy-650
Toyopearl AF-Chelate-650
Toyopearl AF-Epoxy-650
Toyopearl AF-Formyl-650
Toyopearl AF-Heparin HC-650
Toyopearl AF-Red-650
Toyopearl AF-Tresyl-650
Size Exclusion (SEC)


Toyopearl AF-Blue HC-650 is functionalized with Cibachron Blue F3G-A. It has high dynamic binding capacity for proteins, particularly albumin, and has improved caustic stability and lower dye leakage than traditional agarose materials


Structure:

 


Product Attributes:

 Pore size (mean):  100nm
 Particle size (mean):  65µm (M-grade) 
 Pressure rating:  0.3 MPa
 Shipping buffer:  20% ethanol
 pH stability:  4-9

 

Literature:

Catalog -- Process Media Catalog, Affinity Chromatography

Product Overview -- Toyopearl AF-Blue HC-650M, for one step Affinity Isolation of Kinases, Phosphates, Dehydrogenases, Albumin, Factor II, FactorIX, and Interferon

Operating Conditions and Specifications

Comparative information


Comparison of human serum albumin binding capacity at various pH values for AF-Blue HC-650 and Agarose


Comparative dye leakage study of AF-Blue HC-650M and Agarose Blue @ 25°C (after 24 hours)



 

P/N

Description Resin volume Ligand density Adsorption capacity

 0019688

Toyopearl AF-Blue HC-650M 25mL >16mmol/L >18g/L*

 0019689

Toyopearl AF-Blue HC-650M 100mL >16mmol/L >18g/L*

 0019690

Toyopearl AF-Blue HC-650M 1L >16mmol/L >18g/L*

 0019691

Toyopearl AF-Blue HC-650M 5L >16mmol/L >18g/L*

*Human Serum Albumin (HSA)

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Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp