Home  
  Products  
  Literature/Applications  
  Service/Support  
  Search  
search
Tosoh: The Chemistry of Innovation
Process Media Products
Process Media Products
Ion Exchange (IEC)
Hydrophobic Interaction (HIC)
Affinity (AFC)
Toyopearl AF-Amino-650
Toyopearl AF-Blue HC-650
Toyopearl AF-Carboxy-650
Toyopearl AF-Chelate-650
Toyopearl AF-Epoxy-650
Toyopearl AF-Formyl-650
Toyopearl AF-Heparin HC-650
Toyopearl AF-Red-650
Toyopearl AF-Tresyl-650
Size Exclusion (SEC)


Toyopearl AF-Chelate-650 is derivatized with iminodiacetic acid for immobilized metal affinity chromatography or IMAC.IMAC works primarily by binding to histidine and free cysteine containing sequences of proteins or peptides.


Structure:

 


Product Attributes:

 Pore size (mean):  100nm
 Particle size (mean):  65µm (M-grade) 
 Pressure rating:  0.3 MPa
 Shipping buffer:  20% ethanol
 pH stability:  2-12

 

Literature:

Catalog -- Process Media Catalog, Affinity Chromatography

Operating Conditions and Specifications

 


 

P/N

Description Resin volume Ligand density Adsorption capacity

0014475

Toyopearl AF-Chelate-650M 25mL 25-45meq/L >60g/L*

0019800

Toyopearl AF-Chelate-650M 100mL 25-45meq/L >60g/L*

0014907

Toyopearl AF-Chelate-650M 1L 25-45meq/L >60g/L*

0014908

Toyopearl AF-Chelate-650M 5L 25-45meq/L >60g/L*

*Lysozyme

Printer Friendly Version

Main Process Page
Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp