Toyopearl AF-Epoxy-650 is an activated resin provided in dry form for the immobilization of protein ligands for affinity chromatography. It is used when high densities of low molecular weight molecules need to be attached. It is also useful when a conversion to other special functional groups is required prior to ligand immobilization. For instance, its hydrazide form is very useful for carbohydrates or glycoprotein ligands.

Structure:

Product Attributes:

Pore size (mean):  100nm  Particle size (mean):  65µm (M-grade)   Pressure rating:  0.3 MPa  Shipping condition:  dry  Storage temperature:  ambient

Literature:

Catalog -- Process Media Catalog, Affinity Chromatography

Operating Conditions and Specifications

 

P/N Description Resin quantity Ligand density Adsorption capacity 0043402 Toyopearl AF-Epoxy-650M 5g* 600 - 1000µeq/g >60mg/g** 0008000 Toyopearl AF-Epoxy-650M 10g* 600 - 1000µeq/g >60mg/g** 0008038 Toyopearl AF-Epoxy-650M 100g* 600 - 1000µeq/g >60mg/g** 0018315 Toyopearl AF-Epoxy-650M 1,000g* 600 - 1000µeq/g >60mg/g**

*1g yields approximately 3.5mL
**Measured as amount of test protein coupled per gram of dry gel.