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Tosoh: The Chemistry of Innovation
Process Media Products
Process Media Products
Ion Exchange (IEC)
Hydrophobic Interaction (HIC)
Toyopearl Butyl-600
Toyopearl Butyl-650
Toyopearl Ether-650
Toyopearl Hexyl-650
Toyopearl Phenyl-600
Toyopearl PPG-600
Toyopearl SuperButyl-550
TSKgel Ether-5PW
TSKgel Phenyl-5PW
Affinity (AFC)
Size Exclusion (SEC)


TSKgel Ether-5PW is the highest hydrophilicity of the five HIC ligands offered by TBL. It is useful for the separation of very hydrophobic molecules such as membrane proteins.  It is typically used for high performance chromatography and polishing steps. It has the same selectivity as the larger particle Toyopearl Ether-650 products. It often is used instead of cross-linked dextrans with exclusion limits of 10,000 MW.

 

Structure:

 TSKgel Ether-5PW chemical structure


Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

 

Product Attributes:

 Pore size (mean):  100nm
 Particle size (mean):  20µm 
 30µm
 Pressure rating:  2 MPa
 Shipping buffer:  20% ethanol
 pH stability:  1-13

 

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Operating Conditions and Specifications

 Comparative information for HIC resins


Purification of mAbs from ascites fluid on
TSKgel Ether-5PW and Toyopearl Ether-650M

Comparison of Ether-5PW and Toyopearl Ether resins


 
P/N Description Container size Particle size
 0043276 TSKgel Ether-5PW (20) 25mL 10-30µm
 0016052 TSKgel Ether-5PW (20) 100mL 10-30µm
 0016053 TSKgel Ether-5PW (20) 1L 10-30µm
 0018437 TSKgel Ether-5PW (20) 5L 10-30µm
 0043176 TSKgel Ether-5PW (30) 25mL 20-40µm
 0016050 TSKgel Ether-5PW (30) 100mL 20-40µm
 0016051 TSKgel Ether-5PW (30) 1L 20-40µm
 0018439 TSKgel Ether-5PW (30) 5L 20-40µm


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Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp