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Tosoh: The Chemistry of Innovation
Process Media Products
Process Media Products
Ion Exchange (IEC)
Hydrophobic Interaction (HIC)
Toyopearl Butyl-600
Toyopearl Butyl-650
Toyopearl Ether-650
Toyopearl Hexyl-650
Toyopearl Phenyl-600
Toyopearl PPG-600
Toyopearl SuperButyl-550
TSKgel Ether-5PW
TSKgel Phenyl-5PW
Affinity (AFC)
Size Exclusion (SEC)


Toyopearl Phenyl-650 is the middle hydrophobicity of the five HIC ligands offered by TBL. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in intermediate purification and polishing steps. For high performance separations, the use of TSK-GEL Phenyl-5PW is suggested. Both products have the same selectivity.

 

Structure:

 Toyopearl Phenyl-650 chemical structure


Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

 

Product Attributes:

 Pore size (mean):  100nm
 Particle size (mean):  100µm (C-grade)
 65µm (M-grade)
 35µm (S-grade)
 Pressure rating:  0.3 MPa
 Shipping buffer:  20% ethanol
 pH stability:  1-13

 

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Operating Conditions and Specifications

 Comparative information for HIC resins


 Typical Dynamic Binding Capacities for Lysozyme

 Typical Dynamic Binding Capacities for Lysozyme

 Selectivities of Toyopearl HIC resins

  Selectivities of Toyopearl HIC resins

 


 
P/N Description Container size Particle size
0043152 Toyopearl Phenyl-650S 25mL 20-50µm
0014477 Toyopearl Phenyl-650S 100mL 20-50µm
0014784 Toyopearl Phenyl-650S 1L 20-50µm
0014935 Toyopearl Phenyl-650S 5L 20-50µm
0019818 Toyopearl Phenyl-650M 25mL 40-90µm
0014478 Toyopearl Phenyl-650M 100mL 40-90µm
0014783 Toyopearl Phenyl-650M 1L 40-90µm
0014943 Toyopearl Phenyl-650M 5L 40-90µm
0043126 Toyopearl Phenyl-650C 25mL 50-150µm
0014479 Toyopearl Phenyl-650C 100mL 50-150µm
0014785 Toyopearl Phenyl-650C 1L 50-150µm
0014944 Toyopearl Phenyl-650C 5L 50-150µm


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Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp