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Tosoh: The Chemistry of Innovation
Process Media Products
Process Media Products
Ion Exchange (IEC)
Toyopearl CM-650
Toyopearl DEAE-650
Toyopearl GigaCap CM-650
Toyopearl GigaCap Q-650
Toyopearl GigaCap S-650
Toyopearl MegaCap II SP-550EC
Toyopearl QAE-550
Toyopearl SP-550
Toyopearl SP-650
Toyopearl SuperQ-650
TSKgel DEAE-5PW
TSKgel SP-5PW
TSKgel SuperQ-5PW
Hydrophobic Interaction (HIC)
Affinity (AFC)
Size Exclusion (SEC)


Toyopearl SP-650 is a strong cation exchanger for biomolecule purification.  It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed the smaller particle TSKgel SP-5PW should be used. Selectivity remains the same.


Structure:

 


Product Attributes:

 Pore size (mean):  100nm
 Particle size (mean):  100µm (C-grade)
 65µm (M-grade) 
 35µm (S-grade)
 Pressure rating:  0.3 MPa
 Shipping buffer:  20% ethanol
 pH stability:  2-13


Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Operating Conditions and Specifications

Comparative information for CIEX resins


  Influence of pore size on Lysozyme DBC for
  Toyopearl cation exchangers @ 100cm/hour


  pKa's for Ion Exchange groups

pKa's for IEX resins


 
   P/N Description Resin volume Particle size
  0007994 Toyopearl SP-650C 250mL 50-150µm
  0014700 Toyopearl SP-650C 1L 50-150µm
  0007995 Toyopearl SP-650C 5L 50-150µm
  0043202 Toyopearl SP-650M 100mL 40-90µm
  0007997 Toyopearl SP-650M 250mL 40-90µm
  0014699 Toyopearl SP-650M 1L 40-90µm
  0007998 Toyopearl SP-650M 5L 40-90µm
  0019822 Toyopearl SP-650S 25mL 20-50µm
  0008437 Toyopearl SP-650S 250mL 20-50µm
  0014698 Toyopearl SP-650S 1L 20-50µm
  0008438 Toyopearl SP-650S 5L 20-50µm


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Tosoh Bioscience Japan, Shiba, Minato-Ku, Tokyo 105-8623, Japan TEL:+81-3-5427-5118 FAX:+81-3-5427-5198 Email:hlc@tosoh.co.jp