TSKgel SW column

TSKgel SW Columns for Protein Analysis

TSKgel SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.

TSKgel SW-type columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes.

The calibration curves are shown for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SW-type columns. The table summarizes the molecular weight ranges for TSKgel G2000SW, G3000SW and G4000SW columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores. For comparative purposes, similar data is provided as well for TSKgel SWXL and SuperSW-type columns.

Literature :

• Catalog

• SEC Column Products Table

• Instruction Manual

Base Material:	Silica
Particle Size (mean):	10 µm and 13 µm
Pore size (mean):	12.5 nm
Functional Group:	Diol
pH Stability:	2.5 - 7.5
Calibration Range (globular proteins):	5,000 - 100,000 Da
Calibration Range (dextrans):	1,000 - 3 × 10⁴
Calibration Range (polyethylene glycols and oxides):	500 - 15,000

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0005788	TSKgel G2000SW	10μm	Stainless Steel	7.5	30
0005102	TSKgel G2000SW	10μm	Stainless Steel	7.5	60
0005371	Guard column for P/N 0005788 and 0005102		Stainless Steel	7.5	7.5
0006727	TSKgel G2000SW	13μm	Stainless Steel	21.5	30
0005146	TSKgel G2000SW	13μm	Stainless Steel	21.5	60
0005758	Guard Column for P/N 0006727 and 0005146		Stainless Steel	21.5	7.5
0006819	TSKgel SW Top-Off for 7.5mm ID SW, 1g	10μm

Base Material:	Silica
Particle Size (mean):	10 µm and 13 µm
Pore Size (mean):	25 nm
Functional Group:	Diol
pH Stability:	2.5 - 7.5
Calibration Range (globular proteins):	10,000 - 500,000
Calibration Range (dextrans):	2,000 - 7 x 10⁴
Calibration Range (polyethylene glycols and oxides)	1,000 - 3.5 x 10⁴

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0008800	TSKgel G3000SW	10μm	Glass	8	30
0008805	Guard Column for P/N 0008800		Glass	8	4
0005789	TSKgel G3000SW	10μm	Stainless Steel	7.5	30
0005103	TSKgel G3000SW	10μm	Stainless Steel	7.5	60
0005371	Guard Column for P/Ns 0005789, 0005103		Stainless Steel	7.5	7.5
0006819	TSKgel Top-Off for 7.5mmID SW, 1 g	10μm	Stainless Steel
0006728	TSKgel G3000SW	13μm	Stainless Steel	21.5	30
0005147	TSKgel G3000SW	13μm	Stainless Steel	21.5	60
0005758	Guard Column for P/Ns 0006728, 0005147		Stainless Steel	21.5	7.5

Base Material:	Silica
Particle Size (mean):	13 µm and 17 µm
Pore Size (mean):	45 nm
Functional Group:	Diol
pH Stability:	2.5 - 7.5
Calibration Range (globular proteins):	20,000 - 7 × 10⁶
Calibration Range (dextrans):	4,000 - 5 x 10⁵
Calibration Range (polyethylene glycols and oxides):	2,000 - 2.5 x 10⁵

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0008801	TSKgel G4000SW	13μm	Glass	8	30
0008805	Guard column for P/N 0008801		Glass	8	4
0005790	TSKgel G4000SW	13μm	Stainless Steel	7.5	30
0005104	TSKgel G4000SW	13μm	Stainless Steel	7.5	60
0005371	Guard column for P/N 0005790 and 0005104		Stainless Steel	7.5	7.5
0006819	TSKgel SW Top-Off, 1g for P/N 005790, 0005104	10μm
0006729	TSKgel G4000SW	17μm	Stainless Steel	21.5	30
0005148	TSKgel G4000SW	17μm	Stainless Steel	21.5	60
0005758	Guard column for P/N 0006729, 0005148		Stainless Steel	21.5	7.5