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Hydrophobic Interaction Chromatography

疏水色谱(Hydrophobic Interaction Chromatography: HIC)是利用蛋白表面疏水区域和固定相弱疏水性基团之间的相互作用进行层析分离。

HIC是离子交换和尺寸排阻色谱法的很好补充,特别是对于离子交换色谱法难以分离的结构异构体、聚集体等可以通过HIC有效分离。另外,核酸和糖类等亲水性杂质不会被HIC填料吸附,所以能够很好地被分离出来。

即使在高盐浓度条件下也能吸附各种蛋白质。

TOYOPEARL HIC填料的疏水性强弱顺序为:Ether-650 < PPG-600 < Phenyl-650 < Butyl-650、 Phenyl-600、Butyl-600、SuperButyl-550 < Hexyl-650。

 

TOYOPEARL and TSKgel Ether hydrophobic interaction chromatography (HIC) resins have the highest hydrophilicity (lowest hydrophobicity) of the HIC ligands offered by Tosoh Bioscience. The Ether ligand is useful for very hydrophobic molecules such as membrane proteins. It is typically used in intermediate purification and polishing steps. For high performance separations, the use of TSKgel Ether-5PW media is suggested. Both TOYOPEARL and TSKgel Ether products have the same selectivity.

The following Ether HIC resins are currently available from Tosoh Bioscience (available grades noted):

 TOYOPEARL Ether-650 (M, S grades) TSKgel Ether-5PW (20 and 30 micron)
 

TOYOPEARL PPG (polypropylene glycol) hydrophobic interaction chromatography (HIC) resin has the second highest hydrophilicity (second lowest hydrophobicity) of the HIC ligands offered by Tosoh Bioscience. It has been optimized for the purification of monoclonal antibodies and is used as an intermediate purification or polishing step. It has extremely good capacity and recovery for mAbs.

 

The following PPG HIC resin is currently available from Tosoh Bioscience (available grades noted):
 TOYOPEARL PPG-600 ( M-grade only)

TOYOPEARL and TSKgel Phenyl hydrophobic interaction chromatography resins are located in the middle of the hydrophobicity range of the five HIC ligands offered by Tosoh Bioscience. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in intermediate purification and polishing steps. For high performance separations, the use of TSKgel Phenyl-5PW media is suggested. Both TOYOPEARL and TSKgel resin products have the same selectivity.

The following Phenyl HIC resins are currently available from Tosoh Bioscience (available grades noted):

 TOYOPEARL Phenyl-650 (C,M, and S-grades) TSKgel Phenyl-5PW (20 and 30 micron)
 TOYOPEARL Phenyl-600 (M-grade only) TOYOPEARL Phenyl FT-750 (F-grade)

TOYOPEARL Butyl hydrophobic interaction chromatography (HIC) resins have the second highest hydrophobicity of the HIC ligands offered by Tosoh Bioscience. There are three pore sizes available for this ligand and each has been optimized for a specific molecular size profile. The primary applications for TOYOPEARL Butyl resins are the separation of proteins, their isoforms, and aggregate removal. Butyl resins are typically used in intermediate purification and polishing steps, though they can also be used for capture of your target molecule. 

The following Butyl HIC resins are currently available from Tosoh Bioscience (available grades noted):

TOYOPEARL Butyl-650 (C,M, and S-grades) TOYOPEARL Butyl-600 (M-grade only)
TOYOPEARL SuperButyl-550 (C-grade only)  

 

TOYOPEARL Hexyl hydrophobic interaction chromatography (HIC) resin has the highest hydrophobicity of the HIC ligands offered by Tosoh Bioscience. It is used for very hydrophilic proteins or where a low salt elution environment is required. It is useful as an early capture step or for intermediate purification.

The following Hexyl HIC resin is currently available from Tosoh Bioscience (available grades noted):

TOYOPEARL Hexyl-650 (C-grade only)