Affinity Chromatography

亲和色谱(Affinity Chromatography: AFC)是利用生物体内的生物特异性相互作用(例如:酶/底物、抗体/抗原、激素/受体蛋白质等)的分离模式。通过将生物特异性相互作用中的一种物质(称为配基)导入固定相,可以对另一种物质进行吸附。基于这种原理,AFC可对样品进行高选择性分离。

东曹生命科学提供的AFC填料大致分为两种类型。1)已经将配基导入固定相中,可马上进行分离分析的类型;2)在固定相中导入了活化型的官能团,使用者自己固定配基后使用的类型。

前一种类型有抗体特异性亲和填料TOYOPEARL® AF-rProteinA系列和基因特异性亲和填料AF-Blue HC-650、AF-Red-650、AF-Chelate-650以及AF-Heparin HC-650。

后者活化型亲和填料有TOYOPEARL AF-Epoxy-650、AF-Amino-650、AF-Formyl-650、AF-Carboxy-650、AF-Tresyl-650。

 

 

The  following TOYOPEARL  Protein L resins are currently available from Tosoh Bioscience:

TOYOPEARL AF-rProtein L-650F

TOYOPEARL rProtein A resins are functionalized with recombinant Protein A that is expressed in E. coli as opposed to the native protein that is extracted from S. aureus. This recombinant ligand is free of animal derived products and is bound to the TOYOPEARL HW-65F base bead via multi-point attachment. 

The following TOYOPEARL Protein A resins are currently available from Tosoh Bioscience:

TOYOPEARL AF-rProtein A-650F TOYOPEARL AF-rProtein A HC-650F

TOYOPEARL Activated affinity resins are shipped ready for coupling to your ligand of choice directly out of the bottle. 

The following TOYOPEARL Activated Affinity resins are currently available from Tosoh Bioscience: 

TOYOPEARL AF-Tresyl-650M TOYOPEARL AF-Epoxy-650M

TOYOPEARL Reactive Affinity Resins require activation prior to ligand attachment.

The following TOYOPEARL Reactive Affinity resins are currently available from Tosoh Bioscience:

TOYOPEARL AF-Amino-650M TOYOPEARL AF-Carboxy-650M
TOYOPEARL AF-Formyl-650M  

TOYOPEARL Group Specific affinity resins are functionalized with ligands that bind specific functional groups on your target of interest. 

The following TOYOPEARL Group Specific affinity resins are currently available from Tosoh Bioscience:

TOYOPEARL AF-Blue HC-650M TOYOPEARL AF-Red-650M
TOYOPEARL AF-Chelate-650M TOYOPEARL AF-Heparin HC-650M