产品

Size Exclusion Chromatography


东曹生命科学提供多种 不同孔径和粒径的TOYOPEARL HW系列和TSKgel PW系列尺寸排阻层析填料。尺寸排阻层析是根据分子的尺寸大小进行分离的一种层析方式,因此其对分子没有吸附能力。SEC通常不用于层析工艺中的捕获阶段或中间纯化步骤。有时它可用作最后的抛光精制阶段,即将目标蛋白从其他聚合物或其他分子量显著不同的杂质中分离出来。

此外,TOYOPEARL  HW系列填料在经过不同的配基修饰后,可制成离子交换填料(IEX)、疏水相互作用填料(HIC)以及亲和填料(AFC)。因此,其他分离模式的填料也继承了其优良的孔径、粒径及物理化学性质。

 

 

TOYOPEARL HW 的校准曲线

 
 不同粒径的TOYOPEARL HW-55填料的分辨率比较

TOYOPEARL HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.

TOYOPEARL HW-40 resin is available in three particle sizes.  

Pore size (mean) 5 nm
Exclusion limit 10,000 Da
Particle size (mean)   75 µm (C-grade) 
45 µm (F-grade)
30 µm (S-grade)
Pressure rating 0.3 MPa
Shipping buffer 20% ethanol
pH stability 2 - 13

P/N Description Resin volume Particle size
  0007450 Toyopearl HW-40EC 500 mL 100 - 300 µm
  0007449 Toyopearl HW-40C 500 mL 50 - 100 µm
  0014683 Toyopearl HW-40C 1 L 50 - 100 µm
  0007969 Toyopearl HW-40C 5 L 50 - 100 µm
  0007448 Toyopearl HW-40F 500 mL 30 - 60 µm
  0014682 Toyopearl HW-40F 1 L 30 - 60 µm
  0007968 Toyopearl HW-40F 5 L 30 - 60 µm
  0007447 Toyopearl HW-40S 500 mL 20 - 40 µm
  0014681 Toyopearl HW-40S 1 L 20 - 40 µm
  0007967 Toyopearl HW-40S 5 L 20 - 40 µm

TOYOPEARL HW-50 resin is a hydroxylated methacrylic polymer for size exclusion chromatography of proteins between 500-80,000 Da.

TOYOPEARL HW-50 resin is available in two particle sizes.

Pore size (mean) 12.5 nm
Exclusion limit 80,000 Da
Particle size (mean)   45 µm (F-grade) 
30 µm (S-grade)
Pressure rating 0.3 MPa
Shipping buffer 20% ethanol
pH stability 2 - 13

P/N Description Resin volume Particle size
  0007453 Toyopearl HW-50F 500 mL 30 - 60 µm
  0014685 Toyopearl HW-50F 1 L 30 - 60 µm
  0008060 Toyopearl HW-50F 5 L 30 - 60 µm
  0007452 Toyopearl HW-50S 500 mL 20 - 40 µm
  0014684 Toyopearl HW-50S 1 L 20 - 40 µm
  0008059 Toyopearl HW-50S 5 L 20 - 40 µm

TOYOPEARL HW-55 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 1,000-700,000 Da. It also is the base bead for all TOYOPEARL "-550" designated products.

TOYOPEARL HW-55 resin is available in two particle sizes.

Pore size (mean) 50 nm
Exclusion limit  700,000 Da
Particle size (mean)   45 µm (F-grade) 
30 µm (S-grade)
Pressure rating 0.3 MPa
Shipping buffer 20% ethanol
pH stability 2 - 13

P/N Description Resin volume Particle size
  0007457 Toyopearl HW-55F 500 mL 30 - 60 µm
  0014687 Toyopearl HW-55F 1 L 30 - 60 µm
  0008063 Toyopearl HW-55F 5 L 30 - 60 µm
  0007456 Toyopearl HW-55S 500 mL 20 - 40 µm
  0014686 Toyopearl HW-55S 1 L 20 - 40 µm
  0008062 Toyopearl HW-55S 5 L 20 - 40 µm

TOYOPEARL HW-65 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 40,000-5,000,000 Da. It also is the base bead for all TOYOPEARL "-650" designated products.

TOYOPEARL HW-65 SEC resin is available in two particle sizes. 

Pore size (mean) 100 nm
Exclusion limit 5,000,000 Da
Particle size (mean)   45 µm (F-grade) 
30 µm (S-grade)
Pressure rating 0.3 MPa
Shipping buffer 20% ethanol
pH stability 2 - 13

P/N Description Resin volume Particle size
  0007465 Toyopearl HW-65F 500 mL 30 - 60 µm
  0014689 Toyopearl HW-65F 1 L 30 - 60 µm
  0008069 Toyopearl HW-65F 5 L 30 - 60 µm
  0007464 Toyopearl HW-65S 500 mL 20 - 40 µm
  0014688 Toyopearl HW-65S 1 L 20 - 40 µm
  0008068 Toyopearl HW-65S 5 L 20 - 40 µm

TOYOPEARL HW-75 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 500,000-50,000,000 Da.

TOYOPEARL HW-75 resin is available in two particle sizes.

Pore size (mean) > 100 nm
Exclusion limit 50,000,000 Da
Particle size (mean)   45 µm (F-grade) 
30 µm (S-grade)
Pressure rating 0.3 MPa
Shipping buffer  20% ethanol
pH stability 2 - 13

P/N Description Resin volume Particle size
  0007469 Toyopearl HW-75F 500 mL 30 - 60 µm
  0014691 Toyopearl HW-75F 1 L 30 - 60 µm
  0008072 Toyopearl HW-75F 5 L 30 - 60 µm
  0007468 Toyopearl HW-75S 500 mL 20 - 40 µm
  0008071 Toyopearl HW-75S 5 L 20 - 40 µm