Process Media Modes

The science of bio-purification is one of the fastest growing technologies in the world. Companies that accept the pace and are flexible will win.

At Tosoh, we engineer: particle size, pore size, surface area, ligand chemistry, and innovation.

From the research laboratory to full scale manufacturing, we offer the same polymer chemistries in our TSKgel and TOYOPEARL products. Whether you are scaling up from a TSKgel column HPLC method to TOYOPEARL or TSKgel for manufacturing, or are scaling down from a large scale purification to the corresponding TSKgel column for the QC of your target, we make it easy to develop methods to do both.

Tosoh Bioscience currently has process chromatography media available in five modes to suit all of your purification needs:

TOYOPEARL polymeric SEC materials are available in a number of pore sizes and particle diameters. With some limited exceptions, and certain applications such as desalting, SEC is not typically used at process scale. Using molecular size as the only separation mechanism, and not adsorption, eliminates the possibility of any binding capacity for the resin. This makes SEC an inefficient technique for manufacturing.

However the pore sizes, particle sizes, and physical properties of these beads are important to understand, as they become the base beads for our IEX, HIC, and AFC products.

Select a Size Exclusion Resin »

TOYOPEARL and TSKgel polymeric ion exchange resins utilize an aqueous buffer solution whose pH is between the pI of the target protein and the pKa of the resin. Protein elution occurs by either increasing the salt strength of the buffer or a change in the buffer pH itself during the run. The strong ionic interactions give IEC resins very high dynamic binding capacities (DBC). This makes them ideal for any stage in the purification process but more often used for the Capture step.

Select an Ion Exchange Resin »

TOYOPEARL and TSKgel polymeric HIC resins utilize a decreasing salt gradient buffer system for elution. A high initial salt concentration promotes binding between a protein's surface hydrophobic patches and the resin's hydrophobic ligand. Resin ligand density is less than a reversed phase resin. This allows an organic solvent free elution mechanism with high mass and biological activity recoveries. Tosoh's advanced resin design has created HIC resins with DBCs approaching those of reversed phase materials, with developers frequently using them for Intermediate and Polishing purification steps.

Select a Hydrophobic Interaction Chromatography Resin »

TOYOPEARL polymeric AFC resins are available in a variety of group specific ligands. In addition, activated and reactive forms are available for the immobilization of target specific ligands. In AFC the mechanism of binding and elution varies depending on the immobilized ligand used. In some cases such as with TOYOPEARL AF-Chelate-650M (for Immobilized Metal Affinity Chromatography or IMAC) the binding and elution mechanism may change through the use of different metals or chemical modifiers. DBC values for AFC materials vary with ligand and are typically on the low side. However, because AFC has high selectivity and most of the capacity is used for the target, with feedstock impurities remaining unbound, AFC resins are typically used for the Capture and Intermediate purification steps of a manufacturing process.

Select an Affinity Resin »

TOYOPEARL polymeric Mixed Mode Chromatography resins have both ionic and hydrophobic groups in their composition. These unique ligands allow the separation of acidic, basic, and neutral proteins by one resin. Unlike with traditional ion exchange resins, elevated conductivity feedstocks and buffers can be used without diminishing binding capacity.

Select a Mixed Mode Resin »