TOYOPEARL AF-Amino-650M resin may be used to couple ligands using their carboxyl groups (peptide bond formation) or aldehyde groups (reductive amination). Aldehyde groups may be present in a carbohydrate or glycoprotein ligand or may be introduced into the ligand by mild, periodate oxidation.

The optimized functional group density of TOYOPEARL AF-Amino-650M (100 μmol/mL) is ideal for coupling of either proteins or low molecular weight ligands. For example, lactose was coupled by reductive alkylation to yield a ligand density of ca. 30 μmol/mL resin.

Coupling Procedure


Literature :

 • Catalog

 • Instruction Manual