Anion Exchange Chromatography
Anion exchange chromatography is practiced with either a strong or a weak anion exchange column, containing a quarternary ammonium ion, or with a weak anion exchanger, having either a tertiary or secondairy amine functional group, such as DEAE (diethylaminoethyl). A counter ion, often Cl- maintains electroneutrality.
In ion exchange chromatography, the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For instance, in anion exchange chromatography a molecule with a pI of 6.8 is run in a mobile phase buffer at pH 8.0 with the solid support pKa at 10.3.
Highlights:
- Pore structure and bonding chemistry of TSKgel BioAssist Q columns provides high capacity for small to very large molecular weight proteins and nucleic acids
- TSKgel SuperQ-5PW columns have higher capacity than TSKgel DEAE-5PW due to novel bonding chemistry; effective pore size is smaller for TSKgel SuperQ-5PW
- Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is roughly double that of the 5PW packings due to the smaller pore size and larger surface area.
- TSKgel DEAE-5PW and DEAE-2SW columns are available in 2 mm ID format
- Specialty columns for analysis of mono- and disaccharides, sugar alcohols, and organic acids.
- TSKgel DNA-NPR columns for PCR fragment analysis
- TSKgel BioAssist columns are packed in PEEK hardware, while other columns are available in glass and stainless steel.
- Very efficient chromatography can be achieved with the TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores.
Speciality Columns
Oligonucleotide fragments, PCR reagents (TSKgel DNA-NPR)
Mono- and disaccharides (TSKgel Sugar AXI and TSKgel Sugar AXG)