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Cation Exchange Chromatography

Cation Exchange Chromatography

Cation exchange chromatography is practiced with either a strong or a weak cation exchange column, containing a sulfonium ion, or with a weak cation exchanger, having usually a carboxymethyl (CM) functional group. A counter ion, often Na+ maintains electroneutrality.

In ion exchange chromatography the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For example, a molecule with a pI of 8.2 is run in a mobile phase buffer at pH 6.0 with the solid support pKa at 1.2 in cation exchange chromatography.

Highlights:
  • Pore structure and bonding chemistry of TSKgel BioAssist S provides high capacity for medium to large molecular weight proteins.
  • TSKgel BioAssist columns are packed in 4.6 mm ID or 10 mm ID PEEK hardware. Other columns are available in glass and stainless steel for analytic, semi-preparative and preparative applications.
  • Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is approximately double that of the 5PW packings due to the smaller pore size and larger surface area.
  • TSKgel SP-5PW column available in 2 mm ID format.
  • Very efficient chromatography can be achieved with the TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores. 

Specialty columns:

Organic acids by ion-exclusion (TSKgel OApak-A and TSKgel SCX)

Literature:

 • Separation Report No. 100

 • Catalog

 • IEC Column Products Table


Base Material: polymethacrylate
Particle Size (mean):   7 µm and 13 µm
Pore Size (mean): 130 nm
Functional Group: sulfopropyl
Counter Ion: Na+
pH Range: 2.0 - 12.0
Protein Capacity : 70 g gamme globulin/L
 
Small Ion Capacity: 0.1 eq/L
pKa: 2.4

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0019686
TSKgel BioAssist S
7 µm
PEEK 4.6 5
0021411
TSKgel BioAssist S
13 µm
PEEK 10 10

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: spherical silica
Particle Size (mean):   5 µm
Pore Size (mean): 12.5 nm
Functional Group: -CH2COO-
Counter Ion: Na+
pH Range: 2.0 - 7.5
Protein Capacity : ND
 
Small Ion Capacity: > 0.3 meq/g
pKa: 4.2

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0007167
TSKgel CM-2SW
5 µm
Stainless Steel 4.6 25
0007650
Guardgel Kit for P/N 0007167
 

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: spherical silica
Particle Size (mean):   10 µm
Pore Size (mean): 25 nm
Functional Group: -CH2COO-
Counter Ion: Na+
pH Range: 2.0 - 7.5
Protrein Capacity : ND  
Small Ion Capacity: > 0.3 meq/g
pKa: 4.2

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0007162
TSKgel CM-3SW
10 µm
Stainless Steel 7.5 7.5
0007650
Guardgel Kit for P/N 0007162
 

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual

 • Instruction Manual for Glass Columns


Base Material: hydroxylated methacrylic polymer
Particle Size (mean):   10 µm and 13 µm
Pore Size (mean): 100 nm
Functional Group: -CH2COO-
Counter Ion: Na+
pH Range: 2.0 - 12.0
Protein Capacity : 45 mg Hb/mL
 
Small Ion Capacity: > 0.1 eq/L
pKa: 4.2

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0013068
TSKgel CM-5PW
10 µm
Stainless Steel 7.5 7.5
0013069
Guardgel Kit for P/N 0013068
 
Stainless Steel
0014021
TSKgel CM-5PW   13 µm Stainless Steel 21.5
15  
0016094
Guardgel Kit for P/N 0014021
  Stainless Steel    
0014010
TSKgel CM-5PW   10 µm Glass 5
5
0014011
TSKgel CM-5PW   10 µm Glass 8 7.5
0014024
Guardgel Kit for P/N 0014011
  Glass
   
0014012
TSKgel CM-5PW  13 µm Glass 20
15
0014468
Guard Column for P/N 0014012
  Glass 20
2

Literature:

 • Separation Report No. 109

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: hydrophilic polymer
Particle Size (mean):   7 µm and 10 µm
Pore Size (mean): non-porous
Functional Group: carboxymethyl
Counter Ion: Na+
pH Range: 3.0 - 10.0
Static Binding Capacity 
(mg Lysozyme/g dry gel):  
ca. 20 (7 µm)
ca. 15 (10 µm)
 
Small Ion Capacity: 100 µeq/g dry gel
pKa: 4.9
Hardware: stainless steel tubing and fittings, PEEK frits  

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0021965
TSKgel CM-STAT
10 µm
Stainless Steel 3 3.5
0021966
TSKgel CM-STAT
7 µm
Stainless Steel 4.6 10

Literature:

 • Catalog

 • IEC Column Products Table


Base Material: hydrophilic polymer
Particle Size (mean):   5 µm
Pore Size (mean): ND
Functional Group: proprietary
Counter Ion: H+
pH Range: 2.0 - 7.5
Small Ion Capacity: > 1.5 eq/L

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0016653
TSKgel OApak-A
5 µm
Stainless Steel 7.8 30
0016654
TSKgel OApak-P Guard column
10 µm
Stainless Steel 6 4

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: PS-DVB
Particle Size (mean):   5 µm
Pore Size (mean): 6.0 nm
Functional Group: sulfonic acid
Counter Ion: H+ and Na+
pH Range: 2 - 12
Small Ion Capacity: > 1.5 eq/L

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0007156
TSKgel SCX (Na+)
5 µm
Stainless Steel 6 15
0007158
TSKgel SCX (H+)
5 µm
Stainless Steel 7.8 30

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: spherical silica
Particle Size (mean):   5 µm
Pore Size (mean): 12.5 nm
Functional Group: sulfopropyl
Counter Ion: Na+
pH Range: 2.0 - 7.5
Protein Capacity
ND  
Small Ion Capacity: > 0.3 eq/L
pKa: 2.2

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0007165
TSKgel SP-2SW
5 µm
Stainless Steel 4.6 25
0007644
Guardgel Kit for P/N 0007165
 
Stainless Steel

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual

 • Instruction Manual for Glass Columns


Base Material: hydroxylated methacrylic polymer
Particle Size (mean):   10, 13, 20 µm
Pore Size (mean): 100 nm
Functional Group: -(CH2)SO3-
Counter Ion: Na+
pH Range: 2.0 - 7.5
Protein Capacity : 40 g Hb/L
 
Small Ion Capacity: > 0.1 eq/L
pKa: 2.3

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0018758
TSKgel SP-5PW
10 µm
Stainless Steel 2 7.5 
0007161
TSKgel SP-5PW
10 µm
Stainless Steel 7.5 7.5
0007211
Guardgel Kit for P/N 0007161
 
Stainless Steel
0007575
TSKgel SP-5PW
13 µm
Stainless Steel 21.5 15
0016093
Guardgel Kit for P/N 0007575
 
Stainless Steel
0013062
TSKgel SP-5PW
10 µm
Glass 5 5
0008803
TSKgel SP-5PW
10 µm
Glass 8 7.5
0008807
Guardgel Kit for P/N 08803
 
Glass
0014017
TSKgel SP-5PW
13 µm
Glass 20 15
0014467
Guardgel Column for P/N 014017
  Glass
20
2

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: hydroxylated methacrylic  polymer
Particle Size (mean):   2.5 µm
Pore Size (mean): non-porous
Functional Group: -(CH2)SO3-
Counter Ion: Na+
pH Range: 2.0 - 12.0
Protein Capacity : 5 mg Hb/mL
 
Small Ion Capacity: > 0.1 eq/L  
pKa: 2.3

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0013076
TSKgel SP-NPR
2.5 µm
Stainless Steel 4.6 3.5
0021942
TSKgel SP-NPR
2.5 µm
Stainless Steel 0.3 5

Literature:

 • Separation Report No. 109

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: hydrophilic polymer
Particle Size (mean): 7 µm and 10 µm
Pore Size (mean): non-porous
Functional Group: sulfopropyl
Counter Ion: Na+
pH Range: 3.0 - 10.0
Static Binding Capacity
 (mg Lysozyme/g dry gel):  
ca. 15 (7 µm)
ca. 10 (10 µm)
 
Small Ion Capacity: 23 µeg/g dry gel
 
pKa: 4.0  
Hardware: Stainless Steel tubing & fittings, PEEK frits

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0021963
TSKgel SP-STAT
10 µm
Stainless Steel 3 3.5
0021964
TSKgel SP-STAT
7 µm
Stainless Steel 4.6 10