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TSKgel PW column

TSKgel PW Columns for Analysis of Water-Soluble Polymers

TSKgel PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns.


The main application areas for TSKgel PW columns are the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.


The figure to the left shows the calibration curves for all TSKgel PW-type columns. Suitable solvents for various polymer samples are shown in the table below.

 
                                             
 
Specialty TSKgel PW-type Columns

The mixed-mode column, TSKgel GMPW, has an extended linear calibration range, suitable for samples with a broad MW distribution, as well as unknown samples. TSKgel G2000PW, the larger particle size equivalent of TSKgel G-Oligo-PW, is most suitable for semipreparative and preparative isolation of oligosaccharides. TSKgel G1000PW is the preferred column for analysis of low MW oligomers.

Type of Polymer 

Typical Sample

Suitable Eluent

Nonionic hydrophilic

polyyethylene glycol

soluble starch, methyl cellulose, 

pullulan dextran, hydroxyethyl cellulose 

polyvinyl alcohol, polyacrylamide

distilled water
0.01 mol/L NaOH
20% DMSO
Buffer or salt solution (e.g. 0.1-0.5 mol/L NaNO3)

Nonionic Hydrophobic

polyvinylpyrrolidone

Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3)

Anionic Hydrophilic

sodium chondroitin sulfate, sodium alginate, carboymethyl cellulose, sodium polyacrylate, sodium hyaluronate

Buffer or salt solution (e.g. 0.1 mol/L NaNO3)

Anionic Hydrophobic

sulfonated lignin sodium salt,
sodium polystyrenesulfonate

Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3)

Cationic Hydrophilic

glycol chitosan, DEAE-dextran, poly(ethyleneimine), poly(trimethylaminoethyl methacrylate) iodide salt

0.5M acetic acid with 0.3 mol/L Na2SO4 or 0.8 mol/L NaNO3

Cationic Hydrophobic

poly(4-vinylbenzyltrimethylammonium chloride), poly(N-methyl-2-vinylpyridinium) iodide salt

0.5 mol/L acetic acid with 0.3 mol/L Na2SO4

Amphoteric Hydrophilic

peptides, proteins, poly- and oligosaccharides, DNA, RNA

Buffer or salt solution (e.g. 0.1 mol/L NaNO3)

Amphoteric hydrophobic
 

blue dextran, collagen, gelatin, hydrophobic proteins, hydrophobic peptides

Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3 or 35-45% CH3CN in 0.1% TFA)

Base Material: hydroxylated polymethacylate
Particle Size (mean): 12 µm
Pore size (mean): 12.5 nm
Calibration Range (polyethylene glycols and oxides):   up to 2,000 Da
Max. % Organic solvent: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0005761
TSKgel G2000PW
12 µm
Stainless Steel 7.5 30
0005105
TSKgel G2000PW
12 µm
Stainless Steel 7.5 60
0006763
Guard Column for P/N 0005761 and 0005105
12 µm
Stainless Steel 7.5 7.5
Base Material: hydroxylated polymethacrylate
Particle Size (mean): 12 µm and 17 µm
Pore size (mean): <20 nm
Calibration Range (polyethylene glycols and oxides):  
up to 3,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0008028
TSKgel G2500PW
12 µm
Stainless Steel 7.5 30
0008029
TSKgel G2500PW
12 µm
Stainless Steel 7.5 60
0006762
Guard Column for P/N 0008028, 0008029
12 µm
Stainless Steel 7.5 7.5
0016248
TSKgel G2500PW
17 µm
Stainless Steel 21.5 30
0008030
TSKgel G2500PW
17 µm
Stainless Steel 21.5 60
0006758
Guard Column for P/N 0016248, 0008030
17 µm
Stainless Steel 21.5 7.5
Base Material: hydroxylated polymethacylate
Particle Size (mean): 12 µm and 17 µm
Pore size (mean): 20 nm
Calibration Range (polyethylene glycols and oxides):   up to 50,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm)
Length (cm)
0005762
TSKgel G3000PW
12 µm
Stainless Steel 7.5
30
0005106
TSKgel G3000PW
12 µm
Stainless Steel 7.5
60
0006762
Guard Column for P/N 0005762, 0005106
12 µm
Stainless Steel 7.5
7.5
0016249
TSKgel G3000PW
17 µm
Stainless Steel 21.5
30
0005151
TSKgel G3000PW  17 µm
 Stainless Steel 21.5 60
0006758
Guard Column for P/N 0016249, 0005151
17 µm
Stainless Steel 21.5
7.5
Base Material: hydroxylated polymethacylate
Particle Size (mean): 17 µm
Pore size (mean): 50 nm
Calibration Range (polyethylene glycols and oxides):   up to 300,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm)
Length (cm)
0005763
TSKgel G4000PW
17 µm
Stainless Steel 7.5
30
0005107
TSKgel G4000PW
17 µm
Stainless Steel 7.5
60
0006762
Guard Column for P/N 0005763, 0005107
12 µm
Stainless Steel 7.5
7.5
0016250
TSKgel G4000PW
20 µm
Stainless Steel 21.5
30
0005152 TSKgel G4000PW 20 µm  Stainless Steel 21.5  60
0006758
Guard Column for P/N 0016250, 0005152
17 µm
Stainless Steel 21.5
7.5
Base Material: hydroxylated polymethacylate
Particle Size (mean): 17 µm
Pore size (mean): 100 nm
Calibration Range (polyethylene glycols and oxides):   up to 1,000,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0005764
TSKgel G5000PW
17 µm
Stainless Steel 7.5 30
0005108
TSKgel G5000PW
17 µm
Stainless Steel 7.5 60
0006762
Guard Column for P/N 0005764, 0005108
12 µm
Stainless Steel 7.5 7.5
0016251
TSKgel G5000PW
20 µm
Stainless Steel 21.5 30
0005153
TSKgel G5000PW
20 µm
Stainless Steel 21.5 60
0006758
Guard Column for P/N 0016251, 0005153 17 µm
Stainless Steel 21.5 7.5
Base Material: hydroxylated polymethacylate
Particle Size (mean): 17 µm
Pore size (mean): >100 nm
Calibration Range (polyethylene glycols and oxides):   up to 8,000,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0005765
TSKgel G6000PW
17 µm
Stainless Steel 7.5 30
0005109
TSKgel G6000PW
17 µm
Stainless Steel 7.5 60
0006762
Guard Column for P/N 0005765 and 0005109
12 µm
Stainless Steel 7.5 7.5
0020024
TSKgel BioAssist G6PW
17 µm
PEEK 7.8 30
Base Material: hydroxylated polymethacylate
Particle Size (mean): 17 µm
Pore size (mean): <10 - 100 nm
Calibration Range (polyethylene glycols and oxides):   500 - 8,000,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particlesize
Housingmaterial ID(mm) Length(cm)
0008026
TSKgel GMPW
17 µm
Stainless Steel 7.5 30
0008027
TSKgel GMPW
17 µm
Stainless Steel 7.5 60
0006762
Guard Column for P/N 0008026 and 0008027
12 µm
Stainless Steel 7.5 7.5