TOYOPEARL Sulfate-650



TOYOPEARL Sulfate-650F resin is a strong cation exchange resin that exhibits high salt tolerance.  This resin offers the strongest capture of monoclonal antibody (mAb) aggregates over a wide pH range without losing its binding capacity for mAb. With the use of optimized binding conditions, a dynamic binding capacity of > 120 g/L of mAb can be easily achieved with TOYOPEARL Sulfate-650F. This high binding capacity translates into lower operating costs per gram of antibody produced.

A TOYOPEARL HW-65F polymeric bead has been functionalized with a sulfate (SO4-) group.  The 100 nm pore size of this resin, along with proprietary bonding technology, makes TOYOPEARL Sulfate-650F ideal for applications performed in physiological conditions or for post-protein A removal of aggregates.


Wide Working Range of pH: Flexibility rate


TOYOPEARL Sulfate-650F resin works with a wide pH range

 High Resolution and High Salt Tolerance

  • Greater resolution between monomer and aggregates
  • Highest salt tolerance versus other Fractogel resin on the market

Aggregate Removal


 TOYOPEARL Sulfate-650F
 Column size:  6.6 mm ID × 20 cm (6.8 mL)
 Mobile phase:  A: 0.1 mol/L sodium acetate, pH 5.0
   B: mobile phase A + 1 mol/L NaCl
 Gradient:  0-50% B, 20 CV
   100% B, 5 CV
 Flow rate:  300 cm/hr (4 min residence time)
 Detection:  UV @ 280 nm (mAU),
   conductivity (mS/cm)
 Temperature:  ambient
 Injection vol.:  6.8 mL (10 mg/mL-resin load ratio)
 Sample:  IgG, protein A-purified, aggregated, 10 g/L



Literature :

 • Instruction Manual